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Validation Data Gallery

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  • Western Blot (WB) analysis of C2C12 cells using Phospho-NDRG1 (Thr346) Recombinant antibody (85141-5-RR)

    WB analysis of C2C12 using 85141-5-RR (same clone as 85141-5-PBS)

    Non-treated C2C12 cells and Insulin treated C2C12 cells were subjected to SDS PAGE followed by western blot with 85141-5-RR (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85141-5-PBS in a different storage buffer formulation.

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Phospho-NDRG1 (Thr346) Recombinant antibody, PBS Only

Phospho-NDRG1 (Thr346) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85141-5-PBS
Clone No.250220C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

NDRG1 (Thr346), p NDRG1, p NDRG1 (Thr346), Phospho NDRG1, Phospho NDRG1 (Thr346)

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85141-5-PBS targets Phospho-NDRG1 (Thr346) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name N-myc downstream regulated 1
Calculated molecular weight 43 kDa
Observed molecular weight46 kDa, 48 kDa
GenBank accession numberBC006260
Gene Symbol NDRG1
Gene ID (NCBI) 10397
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92597
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)