• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of HEK-293 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of NIH/3T3 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombi (85804-1-RR)

    WB analysis of HSC-T6 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody, PBS Only

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85804-1-PBS
Clone No.250098F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

P Myosin Light Chain 2 (Thr18/Ser19), Phos Myosin Light Chain 2 (Thr18/Ser19), Phos-Myosin Light Chain 2 (Thr18/Ser19), Phospho-MYL12A (Thr18/Ser19), Phospho-MYL12B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85804-1-PBS targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 12A, regulatory, non-sarcomeric
Calculated molecular weight 171 aa, 20 kDa
Observed molecular weight 19-20 kDa
GenBank accession numberBC016372
Gene Symbol MYL12A
Gene ID (NCBI) 10627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19105
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.