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Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells, PMA treated HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87619-1-RR targets Phospho-MSK1 (Ser376) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 5 |
| Calculated molecular weight | 549aa,62 kDa; 802aa,90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC017187 |
| Gene Symbol | MSK1 |
| Gene ID (NCBI) | 9252 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75582 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitogen- and stress-activated kinase 1 (MSK1) is a protein kinase that plays a crucial role in the regulation of gene expression in response to various stimuli, including mitogens, stress, and inflammatory signals. ERK1/2 or p38 MAPK activate MSK1 by phosphorylating Thr581 and Ser360; this primes the C-terminal domain, which then autophosphorylates Ser376 (and Ser212) to unlock the N-terminal catalytic site. (PMID: 15568999)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MSK1 (Ser376) antibody 87619-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |