Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-12 cells, λ phosphatase treated PC-12 cells |
| Positive IF/ICC detected in | λ phosphatase treated U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | MOB1, Mps One Binder kinase activator-like 1B (yeast) |
| Calculated molecular weight | 216 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC003398 |
| Gene Symbol | MOBKL1B |
| Gene ID (NCBI) | 55233 |
| RRID | AB_2923589 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8S9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-AP | Download protocol |
| WB protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
