• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-MKK7 (Ser271/Thr275) Monoclonal antibody (68050-1-Ig)

    WB analysis using 68050-1-Ig

    Non-treated and Calyculin A treated, λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-MKK7 (Ser271/Thr275) Monoclonal antibody (68050-1-Ig)

    WB analysis using 68050-1-Ig

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Phospho-MKK7 (Ser271/Thr275) Monoclonal antibody

Phospho-MKK7 (Ser271/Thr275) Monoclonal Antibody for WB, ELISA
Cat No. 68050-1-Ig
Clone No.1B4B5

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inλ phosphatase treated HeLa cells, HEK-293 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68050-1-Ig targets Phospho-MKK7 (Ser271/Thr275) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 7
Calculated molecular weight 47 kDa
Observed molecular weight47-52 kDa
GenBank accession numberBC038295
Gene Symbol MKK7
Gene ID (NCBI) 5609
RRIDAB_2918792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)

Protocols

Product Specific Protocols
WB protocol for Phospho-MKK7 (Ser271/Thr275) antibody 68050-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols