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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-MEK6 (Ser207) Recombinant antibody (80064-2-RR)

    WB analysis of HEK-293 using 80064-2-RR (same clone as 80064-2-PBS)

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-MEK6 (Ser207) Recombinant antibody (80064-2-RR)

    WB analysis using 80064-2-RR (same clone as 80064-2-PBS)

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

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Phospho-MEK6 (Ser207) Recombinant antibody, PBS Only

Phospho-MEK6 (Ser207) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80064-2-PBS
Clone No.250293G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80064-2-PBS targets Phospho-MEK6 (Ser207) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 6
Calculated molecular weight 334 aa, 37 kDa
Observed molecular weight38 kDa, 40kDa
GenBank accession numberBC012009
Gene Symbol MEK6
Gene ID (NCBI) 5608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52564
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.