Validation Data Gallery
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Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
28844-1-AP targets Phospho-MAP3K1 (Thr1400) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase kinase 1 |
| Calculated molecular weight | 164 kDa |
| Observed molecular weight | 164 kDa |
| GenBank accession number | NM_005921 |
| Gene Symbol | MAP3K1 |
| Gene ID (NCBI) | 4214 |
| RRID | AB_2918209 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. MAP3K1 is activated by autophosphorylation on Thr-1400 and Thr-1412(Uniprot).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MAP3K1 (Thr1400) antibody 28844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Resveratrol inhibits autophagy against myocardial ischemia-reperfusion injury through the DJ-1/MEKK1/JNK pathway | ||
Cell Death Dis CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer |