Validation Data Gallery
Tested Applications
| Positive WB detected in | PDGF treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
29250-1-AP targets Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase kinase 4 |
| Calculated molecular weight | 399 aa, 44 kDa |
| Observed molecular weight | 44-50 kDa |
| GenBank accession number | BC036032 |
| Gene Symbol | MAP2K4 |
| Gene ID (NCBI) | 6416 |
| RRID | AB_2918258 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MAP2K4 (Ser257/Thr261) antibody 29250-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity | ||
J Inorg Biochem Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis | ||
Bioorg Med Chem Synthesis and biological evaluation of lappaconitine analogues as potential anti-neuroinflammatory agents by side chain modification and scaffold hopping strategy | ||
Exp Cell Res The aberrantly activated AURKB supports and complements the function of AURKA in CALR mutated cells through regulating the cell growth and differentiation |