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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-KAP1 (Ser824) Recombinant monoclonal antib (87309-1-RR)

    WB analysis using 87309-1-RR

    Non-treated NIH/3T3 cells and etoposide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87309-1-RR (Phospho-KAP1 (Ser824) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Phospho-KAP1 (Ser824) Recombinant monoclonal antib (87309-1-RR)

    IF Staining of NIH/3T3 using 87309-1-RR

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Phospho-KAP1 (Ser824) Recombinant monoclonal antib (87309-1-RR)

    IF Staining of NIH/3T3 using 87309-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-KAP1 (Ser824) Recombinant monoclonal antibody

Phospho-KAP1 (Ser824) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87309-1-RR
Clone No.252561H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Phospho KAP1 (Ser824), Phospho-KAP1, Phospho-KAP-1, Phospho-KAP-1 (Ser824), Phospho-KAP1(Ser824)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inetoposide treated NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells, etoposide treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87309-1-RR targets Phospho-KAP1 (Ser824) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 28
Calculated molecular weight 89 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_005762
Gene Symbol KAP1
Gene ID (NCBI) 10155
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. Phospho-KAP1 (Ser824) refers to the phosphorylated form of KAP1 (KRAB-associated protein 1) at Serine 824. This post-translational modification plays a significant role in the regulation of gene expression and chromatin structure.

Protocols

Product Specific Protocols
IF protocol for Phospho-KAP1 (Ser824) antibody 87309-1-RRDownload protocol
WB protocol for Phospho-KAP1 (Ser824) antibody 87309-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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