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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-IRS1 (Ser307) Recombinant antibody (85238-1-RR)

    WB analysis using 85238-1-RR

    Non-treated NIH/3T3 cells and TNF alpha treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-IRS1 (Ser307) Recombinant antibody (85238-1-RR)

    WB analysis using 85238-1-RR

    Non-treated and TNF alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Phospho-IRS1 (Ser307) Recombinant antibody

Phospho-IRS1 (Ser307) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85238-1-RR
Clone No.242124E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, TNF alpha treated HeLa cells, TNF alpha treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name insulin receptor substrate 1
Calculated molecular weight 1242 aa, 132 kDa
Observed molecular weight180 kDa
GenBank accession numberBC053895
Gene Symbol IRS1
Gene ID (NCBI) 3667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35568
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)

Protocols

Product Specific Protocols
WB protocol for Phospho-IRS1 (Ser307) antibody 85238-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols