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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal antibody

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31133-1-AP

Synonyms

CD220, HHF5, INSR, insulin receptor, IR

Validation Data Gallery

Applications:
  • WB analysis of HEK-293T using 31133-1-AP

    WB analysis of HEK-293T using 31133-1-AP

    Non-treated and IGF-1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 31133-1-AP (Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Tested Applications

Positive WB detected inIGF-1 treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

31133-1-AP targets Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name INSR
Observed molecular weight95 kDa
GenBank accession numberBC117172
Gene symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin plays a crucial role in brain functions such as memory improvement and energy metabolism. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. This antibody recognizes the phosphorylation of tyrosine 1150 and 1151 of INSR, as well as the phosphorylation of tyrosine 1135 and 1136 of IGF1R. ((PMID:24434591))

Protocols

Product Specific Protocols
WB protocol for Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody 31133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols