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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-INPPL1 (Tyr986/987) Polyclonal antibody

Phospho-INPPL1 (Tyr986/987) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29574-1-AP

Synonyms

INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2

Validation Data Gallery

Applications:
  • WB analysis using 29574-1-AP

    WB analysis using 29574-1-AP

    Non-treated and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

  • WB analysis using 29574-1-AP

    WB analysis using 29574-1-AP

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

Tested Applications

Positive WB detected inUV treated HeLa cells, UV treated NIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy INPPL1 Ready-To-Use IHC Kit. INPPL1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

29574-1-AP targets Phospho-INPPL1 (Tyr986/987) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate phosphatase-like 1
Calculated molecular weight 1258 aa, 139 kDa
Observed molecular weight150 kDa
GenBank accession numberBC140853
Gene symbol INPPL1
Gene ID (NCBI) 3636
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone. It has also been shown that SHIP2 can be phosphorylated at Tyr-986 in COS-7 cells and Chinese hamster ovary cells overexpressing the insulin receptor. (PMID: 11349134)

Protocols

Product Specific Protocols
WB protocol for Phospho-INPPL1 (Tyr986/987) antibody 29574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols