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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H3 (Ser28) Recombinant monoclonal (87654-1-RR)

    WB analysis using 87654-1-RR (same clone as 87654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Histone H3 (Ser28) Recombinant monoclonal (87654-1-RR)

    WB analysis using 87654-1-RR (same clone as 87654-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.

Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only

Phospho-Histone H3 (Ser28) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87654-1-PBS
Clone No.250369D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87654-1-PBS targets Phospho-Histone H3 (Ser28) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight17 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3745676
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

H3S28ph and H3S10ph appear simultaneously in the early stages of mitosis, starting from prophase I (chromosome condensation), distributed along the chromosomes, and participating in chromosome condensation and separation. H3S28ph, as a marker of cell proliferation and transformation, has important value in tumor research.

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