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Tested Applications
| Positive WB detected in | nocodazole treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86873-1-RR targets Phospho-Histone H2B (Ser32) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 2, H2be |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC005827 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 8349 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
During mitosis, Aurora-B directly binds and phosphorylates Histone H2B at Ser32. (PMID: 29563611)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Histone H2B (Ser32) antibody 86873-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |