Validation Data Gallery
Tested Applications
| Positive WB detected in | UV treated HEK-293 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IF | See 5 publications below |
Product Information
29380-1-AP targets Phospho-Histone H2A.X (Ser139) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | H2A histone family, member X |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC013416 |
| Gene Symbol | Histone H2A.X |
| Gene ID (NCBI) | 3014 |
| RRID | AB_3085345 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16104 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser139 (a form called cH2AX) by the kinases ATM and ATR. The phosphorylation of H2AX at Ser139, resulting in the formation of gamma-H2AX puncta in the nuclei, is an early event in the cellular response to DNA damage. Therefore, phospho-Histone H2A. X (Ser139) is also known as γH2AX. The phosphorylation site of H2AX, Ser139, has also been described as Ser140 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 30106130, PMID:22941631)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Histone H2A.X (Ser139) antibody 29380-1-AP | Download protocol |
| WB protocol for Phospho-Histone H2A.X (Ser139) antibody 29380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy | ||
Eur J Med Chem Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation | ||
BMC Pharmacol Toxicol Panobinostat (LBH589) combined with AM1241 induces cervical cancer cell apoptosis through autophagy pathway | ||
Front Cell Dev Biol DNA damage-induced AIM2 pyroptosis in high glucose-induced proximal tubular epithelial cell | ||
Bioorg Chem FL118: A potential bladder cancer therapeutic compound targeting H2A.X identified through library screening | ||
Int J Biol Macromol Membrane-anchored tissue inhibitor of matrix metalloproteinase (TIMP)-1 promotes cell death in head and neck cancer by inducing DNA damage, accumulating collagen II and disrupting cell survival mechanisms |