Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
82762-11-PBS targets Phospho-HER2/ErbB2 (Tyr877) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Calculated molecular weight | 1255 aa, 138 kDa |
| Observed molecular weight | 185 kDa |
| GenBank accession number | BC156755 |
| Gene Symbol | HER2/ErbB2 |
| Gene ID (NCBI) | 2064 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04626 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)