Validation Data Gallery
Tested Applications
| Positive WB detected in | human Neuregulin-1 treated T-47D cells, human Neuregulin-1 treated MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87205-1-RR targets Phospho-HER2/ErbB2 (Tyr1196) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Calculated molecular weight | 1255 aa, 138 kDa |
| Observed molecular weight | 185 kDa |
| GenBank accession number | NM_004448 |
| Gene Symbol | HER2/ErbB2 |
| Gene ID (NCBI) | 2064 |
| RRID | AB_3745406 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr1196) is a phosphorylated form of the HER2 protein, which is a receptor tyrosine kinase that plays a critical role in cell growth, proliferation, and survival. The phosphorylation of HER2 at Tyr1196 is associated with the activation of downstream signaling pathways. In breast cancer, HER2 is overexpressed and contributes to aggressive tumor growth and poor prognosis. Overexpression of Her2/ErbB2/Neu in cancer is often correlated with recurrent distant metastasis, although the mechanism still remains largely elusive. Tyr1139/Tyr1196 are autonomously phosphorylated, when Her2 is overexpressed. (PMID: 21966491)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-HER2/ErbB2 (Tyr1196) antibody 87205-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |