Validation Data Gallery
Tested Applications
| Positive WB detected in | MG132 treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87445-2-RR targets Phospho-EPHA2 (Tyr588) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | EPH receptor A2 |
| Calculated molecular weight | 976 aa, 108 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC037166 |
| Gene Symbol | EPHA2 |
| Gene ID (NCBI) | 1969 |
| RRID | AB_3745550 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29317 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ephrin type-A receptor 2 (EPHA2), belongs to the receptor tyrosine kinases (RTKs) family, that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (PMID: 11114742). The "reverse signaling" function, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allows interactions with other proteins that may activate signaling pathways in the ligand-expressing cells. The detected molecular weight is around 110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-EPHA2 (Tyr588) antibody 87445-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |