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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR)

    WB analysis of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR)

    FC experiment of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR, Clone:242049G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

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Phospho-EIF4E (Ser209) Recombinant antibody, PBS Only

Phospho-EIF4E (Ser209) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84981-1-PBS
Clone No.242049G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

EIF4E, EIF4F, EIF4EL1, eIF-4E, eIF 4E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84981-1-PBS targets Phospho-EIF4E (Ser209) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E
Calculated molecular weight 29 kDa
Observed molecular weight25 kDa
GenBank accession numberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06730
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)