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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EGFR (Tyr1197) Recombinant antibody (84906-1-RR)

    WB analysis using 84906-1-RR

    Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84906-1-RR (Phospho-EGFR (Tyr1197) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of A431 cells using Phospho-EGFR (Tyr1197) Recombinant antibody (84906-1-RR)

    FC experiment of A431 using 84906-1-RR

    1x10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-EGFR (Tyr1197) Recombinant Antibody (84906-1-RR, Clone:241826G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-EGFR (Tyr1197) Recombinant antibody

Phospho-EGFR (Tyr1197) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84906-1-RR
Clone No.241826G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EGF Receptor (Tyr1173), EGFR (Tyr1197), p-EGF Receptor, p-EGF Receptor (Tyr1173), p-EGFR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84906-1-RR targets Phospho-EGFR (Tyr1197) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight175 kDa
GenBank accession numberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852)

Protocols

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr1197) antibody 84906-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols