• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using Phospho-EGFR (Tyr1016) Recombinant antibody (82736-11-RR)

    WB analysis of A431 using 82736-11-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82736-11-RR (Phospho-EGFR (Tyr1016) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • Schematic diagram of the phosphorylation sites of EGFR.

    Schematic diagram of the phosphorylation sites of EGFR.

    In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-EGFR (Tyr1016) Recombinant monoclonal anti (82736-11-RR)

    IF Staining of A431 using 82736-11-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-EGFR (Tyr1016) antibody (82736-11-RR, Clone: 250687B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using Phospho-EGFR (Tyr1016) Recombinant monoclonal anti (82736-11-RR)

    FC experiment of A431 using 82736-11-RR

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody (82736-11-RR, Clone:250687B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody

Phospho-EGFR (Tyr1016) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82736-11-RR
Clone No.250687B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EGF Receptor (Tyr992), EGFR (Tyr1016), EGFR (Tyr992), p EGFR (Tyr1016), p-EGF Receptor (Tyr992)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inEGF treated A431 cells
Positive IF/ICC detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells

This antibody, also known as EGFR Tyr992, is the site where the signal peptide is removed.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82736-11-RR targets Phospho-EGFR (Tyr1016) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight170 kDa
GenBank accession numberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed.

Protocols

Product Specific Protocols
FC protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RRDownload protocol
IF protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RRDownload protocol
WB protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...