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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-CDK1/CDC2 (Tyr15) Recombinant monoclonal a (82751-5-RR)

    WB analysis of HeLa using 82751-5-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 82751-5-RR (Phospho-CDK1 (Tyr15) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-CDK1/CDC2 (Tyr15) Recombinant monoclonal a (82751-5-RR)

    IF Staining of HeLa using 82751-5-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-CDK1/CDC2 (Tyr15) antibody (82751-5-RR, Clone: 251130A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-CDK1 (Tyr15) Recombinant monoclonal antibo (82751-5-RR)

    FC experiment of HeLa using 82751-5-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-CDK1 (Tyr15) Recombinant monoclonal antibody (82751-5-RR, Clone:251130A5) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-CDK1/CDC2 (Tyr15) Recombinant monoclonal antibody

Phospho-CDK1/CDC2 (Tyr15) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82751-5-RR
Clone No.251130A5
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

cdc2 (Tyr15), CDK1 (Tyr15), p-cdc2, p-cdc2 (Tyr15), p-CDK1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

82751-5-RR targets Phospho-CDK1/CDC2 (Tyr15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cell division cycle 2, G1 to S and G2 to M
Calculated molecular weight 25 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC014563
Gene Symbol CDK1
Gene ID (NCBI) 983
RRIDAB_3743425
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06493
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1. This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578)

Protocols

Product Specific Protocols
FC protocol for Phospho-CDK1/CDC2 (Tyr15) antibody 82751-5-RRDownload protocol
IF protocol for Phospho-CDK1/CDC2 (Tyr15) antibody 82751-5-RRDownload protocol
WB protocol for Phospho-CDK1/CDC2 (Tyr15) antibody 82751-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pharm Biomed Anal

Mechanisms underlying the therapeutic effects of Amygdalin in treating Cervical Cancer based on multi-omics analysis.

Authors - Qianqian Jiang
View Article
humanWB

Cell Biol Int

RPL27 Promotes Gastric Cancer Progression by Enhancing Oxidative Phosphorylation Via FMC1.

Authors - Yan Zheng
View Article
humanWB

Int J Biol Macromol

Flap endonuclease 1 drives tumorigenesis across cancers and is a master regulator of proliferation and genomic instability in liver cancer.

Authors - Fang Li
View Article
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