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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-CAD (Ser1859) Recombinant monoclonal antib (87337-2-RR)

    WB analysis using 87337-2-RR

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-CAD (Ser1859) Recombinant monoclonal antib (87337-2-RR)

    WB analysis using 87337-2-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-CAD (Ser1859) Recombinant monoclonal antib (87337-2-RR)

    WB analysis using 87337-2-RR

    Non-treated MCF-7 cells and Rapamycin treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Phospho-CAD (Ser1859) Recombinant monoclonal antib (87337-2-RR)

    IF Staining of MCF-7 using 87337-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed Rapamycin treated MCF-7 cells using Phospho-CAD (Ser1859) antibody (87337-2-RR, Clone: 252640C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-CAD (Ser1859) Recombinant monoclonal antibody

Phospho-CAD (Ser1859) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87337-2-RR
Clone No.252640C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CAD (Ser1859), p-CAD, p-CAD (Ser1859), Phospho-CAD, CAD

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Rapamycin treated MCF-7 cells
Positive IF/ICC detected inRapamycin treated MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87337-2-RR targets Phospho-CAD (Ser1859) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated molecular weight 2225 aa, 243 kDa
Observed molecular weight250 kDa
GenBank accession numberNM_004341
Gene Symbol CAD
Gene ID (NCBI) 790
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP27708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) refers to the phosphorylated form of the multi-enzyme CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) on serine 1859. This modification is catalyzed by ribosomal protein S6 kinase 1 (S6K1) downstream of mTORC1 signaling and serves as a metabolic switch that converts CAD from its basal state into a highly active oligomer, thereby accelerating the first three rate-limiting steps of de novo pyrimidine synthesis. (PMID:25422319, PMID: 32974014)

Protocols

Product Specific Protocols
IF protocol for Phospho-CAD (Ser1859) antibody 87337-2-RRDownload protocol
WB protocol for Phospho-CAD (Ser1859) antibody 87337-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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