Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87795-3-RR targets Phospho-Beta Arrestin 1 (Ser412) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | arrestin, beta 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | NM_004041 |
| Gene Symbol | Beta Arrestin 1 |
| Gene ID (NCBI) | 408 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49407 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Phospho‑β‑arrestin 1 (Ser412) is a key phosphorylated isoform of β‑arrestin 1. Its Ser412 residue is mainly phosphorylated by ERK, governing GPCR desensitization, receptor internalization and downstream signal transduction. This modification regulates β‑arrestin 1 subcellular localization and scaffold function, participating in cell proliferation and insulin‑related ERK signaling desensitization as a vital negative feedback regulator. (PMID: 10347142, PMID: 15520010)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Beta Arrestin 1 (Ser412) antibody 87795-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |