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Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87327-1-RR targets Phospho-BCR (Tyr177) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | breakpoint cluster region |
| Calculated molecular weight | 143 kDa |
| Observed molecular weight | 210 kDa |
| GenBank accession number | NM_004327 |
| Gene Symbol | BCR |
| Gene ID (NCBI) | 613 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11274 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The aminoterminal BCR-encoded sequences of BCR-ABL contain a tyrosine-phosphorylated site that binds the SH2 domain of the adaptor protein GRB2. It is now evident that the phosphorylation of BCR Tyr177 is essential for BCR-ABL-mediated leukemogenesis, and its mutation largely abolishes GRB2 binding and diminishes BCR-ABL-induced Ras activation. (PMID: 22156549)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-BCR (Tyr177) antibody 87327-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |