Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
80771-2-PBS targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | B-cell CLL/lymphoma 2 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC027258 |
| Gene Symbol | BCL2 |
| Gene ID (NCBI) | 596 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10415 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)
