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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-ATR (Ser428) Recombinant monoclonal antibo (86330-1-RR)

    WB analysis of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Phospho-ATR (Ser428) Recombinant monoclonal antibo (86330-1-RR)

    IF Staining of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86330-1-PBS in a different storage buffer formulation.

Phospho-ATR (Ser428) Recombinant monoclonal antibody, PBS Only

Phospho-ATR (Ser428) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86330-1-PBS
Clone No.250988D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86330-1-PBS targets Phospho-ATR (Ser428) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name ataxia telangiectasia and Rad3 related
Calculated molecular weight 301 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001184
Gene Symbol ATR
Gene ID (NCBI) 545
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13535
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)