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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ASK1 (Ser966) Recombinant monoclonal antib (80142-8-RR)

    WB analysis using 80142-8-RR

    NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80142-8-RR (Phospho-ASK1 (Ser966) antibody) blocked with BSA only, Phospho-ASK1 (Ser966) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of COS-7 cells using Phospho-ASK1 (Ser966) Recombinant monoclonal antib (80142-8-RR)

    WB analysis of COS-7 using 80142-8-RR

    Non-treated COS-7, phosphatase inhibitor treated and λ phosphatase treated COS-7 cells were subjected to SDS PAGE followed by western blot with 80142-8-RR (Phospho-ASK1 (Ser966) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-ASK1 (Ser966) Recombinant monoclonal antibody

Phospho-ASK1 (Ser966) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 80142-8-RR
Clone No.252570F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, ELISA

Synonyms

ASK1 (Ser966), ASK1 (Ser967), p-ASK1, p-ASK1 (Ser966), p-ASK1 (Ser967)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOS-7 cells, NIH/3T3 cells, λ phosphatase treated COS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80142-8-RR targets Phospho-ASK1 (Ser966) in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
Observed molecular weight155 kDa
GenBank accession numberBC054503
Gene Symbol ASK1
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99683
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. The predicted MW of ASK1 is around 155 kDa and the 110-120 kDa fragment has also been reported.

Protocols

Product Specific Protocols
WB protocol for Phospho-ASK1 (Ser966) antibody 80142-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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