Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
87322-1-PBS targets Phospho-ALK (Tyr1507) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | anaplastic lymphoma receptor tyrosine kinase |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC157090 |
| Gene Symbol | ALK |
| Gene ID (NCBI) | 238 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UM73 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Anaplastic lymphoma kinase was first described in 1994 as the NPM-ALK fusion protein that is expressed in the majority of anaplastic large-cell lymphomas. About 2/3rds of anaplastic large-cell lymphomas possess a balanced chromosomal translocation in which the entire nucle-ophosmin (NPM) gene on chromosome 5 is fused to the 3' portion of the ALK gene (including the entire intracellular segment with its protein kinase domain) on chromosome 2. This oncogenic ALK protein kinase is a chimeric protein created by a translocation between chromosomes(2;5)(p23;q35) that generates the NPM-ALK fusion protein. NPM-ALK also known as p80, since the chimeric protein weighs 80 kDa. Studies of NPM-ALK have identified pTyr1096 as the binding site for insulin receptor substrate 1 (IRS1); pTyr1507 is the binding site for Shc and pTyr1604 is the binding site for PLC-Y (native human ALK receptor residue numbers are given; subtract 940 to obtain the NPM-ALK residue). (PMID: 23201355, PMID: 33466277)