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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Thr450) Recombinant monoclonal antibo (87220-1-RR)

    WB analysis using 87220-1-RR

    Non-treated MCF-7 cells, phosphatase inhibitor treated MCF-7 cells and λ phosphatase treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87220-1-RR (Phospho-AKT (Thr450) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-AKT (Thr450) Recombinant monoclonal antibody

Phospho-AKT (Thr450) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87220-1-RR
Clone No.252456B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AKT (phospho T450), AKT1 (phospho T450), p-AKT, p-AKT (Thr450), p-AKT T450

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, λ phosphatase treated MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87220-1-RR targets Phospho-AKT (Thr450) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491)

Protocols

Product Specific Protocols
WB protocol for Phospho-AKT (Thr450) antibody 87220-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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