Phospho-AKT (Ser473) Monoclonal antibody, PBS Only

Non-treated Jurkat and HEK-293 cells and Calyculin A treated Jurkat and HEK-293 cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Non-treated HEK-293T and IGF1 or Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Non-treated HSC-T6 and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Non-treated Jurkat and TPA treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66444-1-Ig (AKT-phospho-S473 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66444-1-Ig (AKT-phospho-S473 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded untreated (left) or Calyculin A treated (right) Jurkat cells slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded untreated (left) or Calyculin A treated (right) Jurkat cells slide using 66444-1-Ig (Phospho-AKT (Ser473) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.

1X10^6 PC-3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-AKT (Ser473) (66444-1-Ig, Clone:1C10B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66444-1-PBS in a different storage buffer formulation.