Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, NIH/3T3 cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 49 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
29119-1-AP targets Phospho-ACC1 (Ser79) in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | acetyl-Coenzyme A carboxylase alpha |
| Calculated molecular weight | 2383 aa, 275 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | BC137287 |
| Gene Symbol | Acetyl-CoA Carboxylase 1 |
| Gene ID (NCBI) | 31 |
| RRID | AB_2918238 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-ACC1 (Ser79) antibody 29119-1-AP | Download protocol |
| IF protocol for Phospho-ACC1 (Ser79) antibody 29119-1-AP | Download protocol |
| WB protocol for Phospho-ACC1 (Ser79) antibody 29119-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Autophagy PRKAB2 as a tumor suppressor in renal cell carcinoma: inhibiting mitophagy via the LRPPRC-PRKN/parkin interaction and cardiolipin biosynthesis. | ||
J Adv Res Sodium citrate targeting Ca2+/CAMKK2 pathway exhibits anti-tumor activity through inducing apoptosis and ferroptosis in ovarian cancer | ||
Acta Pharm Sin B Schisanhenol ameliorates non-alcoholic fatty liver disease via inhibiting miR-802 activation of AMPK-mediated modulation of hepatic lipid metabolism | ||
Sci Adv Liver endothelial zonation orchestrates hepatic steatosis onset through retinoic acid-regulated FGF1. |