Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15294-1-AP (same clone as 15294-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (Perilipin-2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
WB analysis using 15294-1-AP (same clone as 15294-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using Perilipin-2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin-2 antibody (15294-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Perilipin-2 Polyclonal antibody, PBS Only

Perilipin-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15294-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15294-1-PBS targets Perilipin-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7539 Product name: Recombinant human ADRP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-437 aa of BC005127 Sequence: DRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH 相同性解析による交差性が予測される生物種
Full Name	adipose differentiation-related protein
Calculated molecular weight	48 kDa
Observed molecular weight	45-48 kDa
GenBank accession number	BC005127
Gene Symbol	Perilipin-2
Gene ID (NCBI)	123
RRID	AB_2878122
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99541
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. This antibody is not suitable for immunofluorescence staining of frozen sections.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human liver using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)

IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)

IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

Perilipin-2 Polyclonal antibody, PBS Only

Perilipin-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

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