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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Pericentrin Polyclonal antibody (22271-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP (same clone as 22271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of MDCK using 22271-1-AP (same clone as 22271-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of C2C12 using 22271-1-AP (same clone as 22271-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pericentrin Polyclonal antibody (22271-1-AP)

    IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

Pericentrin Polyclonal antibody, PBS Only

Pericentrin Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 22271-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22271-1-PBS targets Pericentrin in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name pericentrin
Calculated molecular weight 378 kDa
GenBank accession numberNM_006031
Gene Symbol Pericentrin
Gene ID (NCBI) 5116
RRIDAB_2879056
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95613
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.

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