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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    WB analysis of HepG2 using 13797-1-AP (same clone as 13797-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    WB analysis of HeLa using 13797-1-AP (same clone as 13797-1-PBS)

    WB result of Pentraxin 3 antibody (13797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    WB analysis using 13797-1-AP (same clone as 13797-1-PBS)

    HUVEC cells lysates were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    WB analysis of HeLa using 13797-1-AP (same clone as 13797-1-PBS)

    WB result of Pentraxin 3 antibody (13797-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    IHC staining of human cervical cancer using 13797-1-AP (same clone as 13797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    IHC staining of human cervical cancer using 13797-1-AP (same clone as 13797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    IF Staining of human placenta using 13797-1-AP (same clone as 13797-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Pentraxin 3 antibody (13797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Pentraxin 3 Polyclonal antibody (13797-1-AP)

    IF Staining of HepG2 using 13797-1-AP (same clone as 13797-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Pentraxin 3 antibody (13797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Pentraxin 3 Polyclonal antibody, PBS Only

Pentraxin 3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 13797-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3, Pentraxin-related protein PTX3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13797-1-PBS targets Pentraxin 3 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4624

Product name: Recombinant human PTX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-381 aa of BC039733

Sequence: DYDLMYVNLDNEIDNGLHPTEDPTPCDCGQEHSEWDKLFIMLENSQMRERMLLQATDDVLRGELQRLREELGRLAESLARPCAPGAPAEARLTSALDELLQATRDAGRRLARMEGAEAQRPEEAGRALAAVLEELRQTRADLHAVQGWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLNKTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELAATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIRGNIVGWGVTEIQPHGGAQYVS

相同性解析による交差性が予測される生物種
Full Name pentraxin-related gene, rapidly induced by IL-1 beta
Calculated molecular weight 381 aa, 42 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC039733
Gene Symbol Pentraxin 3/PTX3
Gene ID (NCBI) 5806
RRIDAB_2165302
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP26022
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif99, the pentraxin domain (PMID: 15771574). Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties (PMID: 15771574). Pentraxin 3 is an acute-phase glycoprotein that plays a role in regulating innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility, and cancer (PMID: 29412047). Pentraxin 3 is a biomarker for cardiovascular disease (PMID: 22347626). Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders (PMID: 22347626; 31057548). In WB, there're two major bands, an upper signal at 80-86 kDa and a lower signal at 42 kDa, representing Pentraxin 3 dimers and monomers, respectively (PMID: 18223257).

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