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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    WB analysis using 86398-1-RR (same clone as 86398-1-PBS)

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 86398-1-RR (Phospho-Tyrosine antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    IF Staining of A431 using 86398-1-RR (same clone as 86398-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hEGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-Tyrosine Recombinant monoclonal antibody (86398-1-RR)

    IF Staining of A431 using 86398-1-RR (same clone as 86398-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hEGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86398-1-PBS in a different storage buffer formulation.

Phospho-Tyrosine Recombinant monoclonal antibody, PBS Only

Phospho-Tyrosine Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86398-1-PBS
Clone No.250495G7

Host / Isotype

Rabbit / IgG

Reactivity

-

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

P Tyr, p Tyrosine, pan phospho, pan-phospho, Pan-phospho-Tyr

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86398-1-PBS targets Phospho-Tyrosine in WB, IF/ICC, Indirect ELISA applications and shows reactivity with - samples.

Tested Reactivity -
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name Phospho-Tyrosine
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.

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