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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    WB analysis using 86370-1-RR (same clone as 86370-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IHC staining of human breast cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IHC staining of human breast cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IF Staining of human lung cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using Pan-Keratin (TypeⅡ) Recombinant antibody (86370-1-RR)

    IF Staining of human cervical cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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Pan-Keratin (TypeⅡ) Recombinant antibody, PBS Only

Pan-Keratin (TypeⅡ) Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86370-1-PBS
Clone No.251040F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86370-1-PBS targets Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Pan-Keratin (TypeⅡ)
Observed molecular weight52-68 kDa
Gene Symbol
Gene ID (NCBI) Pan-Keratin (TypeⅡ)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14533
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.