Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, ROS1728 cells, HSC-T6 cells. RAW 264.7 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 67 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
| IP | See 9 publications below |
| CoIP | See 8 publications below |
Product Information
66289-1-Ig targets Pan Acetylation in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA, Acetylation assay applications and shows reactivity with chemical compound samples.
| Tested Reactivity | chemical compound |
| Cited Reactivity | human, mouse, rat, drosophila, rice |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Pan Acetylation |
| Observed molecular weight | 17 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881672 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pan Acetylation antibody 66289-1-Ig | Download protocol |
| WB protocol for Pan Acetylation antibody 66289-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Disrupting KAT8 Liquid-Liquid Phase Separation with Hybrid Vesicle-Liposome Platform for Enhanced PD-L1 Blockade Treatment. | ||
Cancer Res PRODH2-Mediated Metabolism in the Bone Microenvironment Promotes Breast Cancer Metastasis. | ||
Nat Commun Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis | ||
Nat Commun Multi-protomics analysis identified host cellular pathways perturbed by tick-borne encephalitis virus infection | ||
Nat Commun Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes. | ||
Nat Commun MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation |