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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYROXD2 Recombinant antibody (83954-1-RR)

    WB analysis using 83954-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PYROXD2 Recombinant antibody (83954-1-RR)

    WB analysis using 83954-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83954-1-RR (PYROXD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83954-1-RR

    Affinity and Kinetic Characterization of 83954-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83954-1-RR against Human PYROXD2 were performed. The affinity constant is below 1 pM.

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PYROXD2 Recombinant antibody

PYROXD2 Recombinant Antibody for WB, ELISA
Cat No. 83954-1-RR
Clone No.240984A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C10orf33, YueF, FP3420, 240984A12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, LNCaP cells, U-251 cells, A375 cells, L02 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83954-1-RR targets PYROXD2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PYROXD2 fusion protein Ag14701 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 33
Calculated molecular weight 581 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC006131
Gene Symbol PYROXD2
Gene ID (NCBI) 84795
RRIDAB_3671533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N2H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).

Protocols

Product Specific Protocols
WB protocol for PYROXD2 antibody 83954-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols