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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYROXD2 Polyclonal antibody (21845-1-AP)

    WB analysis using 21845-1-AP (same clone as 21845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21845-1-AP (PYROXD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using PYROXD2 Polyclonal antibody (21845-1-AP)

    IHC staining of rat liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using PYROXD2 Polyclonal antibody (21845-1-AP)

    IHC staining of rat liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PYROXD2 Polyclonal antibody (21845-1-AP)

    IHC staining of mouse liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PYROXD2 Polyclonal antibody (21845-1-AP)

    IHC staining of mouse liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PYROXD2 Polyclonal antibody (21845-1-AP)

    IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PYROXD2 antibody (21845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PYROXD2 Polyclonal antibody (21845-1-AP)

    IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PYROXD2 antibody (21845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

PYROXD2 Polyclonal antibody, PBS Only

PYROXD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21845-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C10orf33, FP3420, YueF

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21845-1-PBS targets PYROXD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15004

Product name: Recombinant human C10orf33 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 232-581 aa of BC006131

Sequence: DQWFESEPLKATLATDAVIGAMTSPHTPGSGYVLLHHVMGGLEGMQGAWGYVQGGMGALSDAIASSATTHGASIFTEKTVAKVQVNSEGCVQGVVLEDGTEVRSKMVLSNTSPQITFLKLTPQEWLPEEFLERISQLDTRSPVTKINVAVDRLPSFLAAPNAPRGQPLPHHQCSIHLNCEDTLLLHQAFEDAMDGLSSHRPVIELCIPSSLDPTLAPPGCHVVSLFTQYTPYTLAGGKAWDEQERDAYADRVFDCIEVYAPGFKDSVVGRDILTPPDLERIFGLPGGNIFHCAMSLDQLYFARPVPLHSGYRCPLQGLYLCGSGAHPGGGVMGAAGRNAAHVAFRDLKSM

相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 33
Calculated molecular weight 581 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC006131
Gene Symbol PYROXD2
Gene ID (NCBI) 84795
RRIDAB_3669377
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2H3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).

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