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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PYROXD1 Recombinant antibody (84289-7-RR)

    WB analysis of HeLa using 84289-7-RR (same clone as 84289-7-PBS)

    WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells. This data was developed using the same antibody clone with 84289-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PYROXD1 Recombinant antibody (84289-7-RR)

    WB analysis using 84289-7-RR (same clone as 84289-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84289-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84289-7-RR

    Affinity and Kinetic Characterization of 84289-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

PYROXD1 Recombinant antibody, PBS Only

PYROXD1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84289-7-PBS
Clone No.241639D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84289-7-PBS targets PYROXD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PYROXD1 fusion protein Ag34768 相同性解析による交差性が予測される生物種
Full Name pyridine nucleotide-disulphide oxidoreductase domain 1
Observed molecular weight55~60 kDa
GenBank accession numberBC021662
Gene Symbol PYROXD1
Gene ID (NCBI) 79912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8WU10
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.