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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PYROXD1 Polyclonal antibody (31093-1-AP)

    WB analysis of HeLa using 31093-1-AP

    WB result of PYROXD1 antibody (31093-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PYROXD1 Polyclonal antibody (31093-1-AP)

    WB analysis using 31093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31093-1-AP (PYROXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

PYROXD1 Polyclonal antibody

PYROXD1 Polyclonal Antibody for WB, ELISA
Cat No. 31093-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

pyridine nucleotide disulphide oxidoreductase domain 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31093-1-AP targets PYROXD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYROXD1 fusion protein Ag34768 相同性解析による交差性が予測される生物種
Full Name pyridine nucleotide-disulphide oxidoreductase domain 1
Observed molecular weight55 kDa
GenBank accession numberBC021662
Gene Symbol PYROXD1
Gene ID (NCBI) 79912
RRIDAB_3669851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU10
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.

Protocols

Product Specific Protocols
WB protocol for PYROXD1 antibody 31093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols