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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYGM-Specific Polyclonal antibody (19716-1-AP)

    WB analysis using 19716-1-AP (same clone as 19716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19716-1-AP (PYGM-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PYGM-Specific Polyclonal antibody (19716-1-AP)

    WB analysis of HepG2 using 19716-1-AP (same clone as 19716-1-PBS)

    WB result of PYGM-Specific antibody (19716-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGM-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PYGM-Specific Polyclonal antibody (19716-1-AP)

    IHC staining of human skeletal muscle using 19716-1-AP (same clone as 19716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PYGM-Specific Polyclonal antibody (19716-1-AP)

    IHC staining of human skeletal muscle using 19716-1-AP (same clone as 19716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PYGM-Specific Polyclonal antibody (19716-1-AP)

    IHC staining of human liver using 19716-1-AP (same clone as 19716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PYGM-Specific Polyclonal antibody (19716-1-AP)

    IHC staining of human liver using 19716-1-AP (same clone as 19716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PYGM-Specific Polyclonal antibody (19716-1-AP)

    IF Staining of HepG2 using 19716-1-AP (same clone as 19716-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PYGM-Specific antibody (19716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19716-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PYGM-Specific Polyclonal antibody, PBS Only

PYGM-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19716-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19716-1-PBS targets PYGM-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphorylase, glycogen, muscle
Calculated molecular weight 97 kDa
Observed molecular weight 97-105 kDa
GenBank accession numberNM_005609
Gene Symbol PYGM
Gene ID (NCBI) 5837
RRIDAB_10642009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11217
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.