Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, U-251 cells, MCF-7 cells, rat brain tissue, fetal human brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86737-3-RR targets PYGB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2732 Product name: Recombinant human PYGB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 544-843 aa of BC017045 Sequence: LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD 相同性解析による交差性が予測される生物種 |
| Full Name | phosphorylase, glycogen; brain |
| Calculated molecular weight | 843 aa, 97 kDa |
| Observed molecular weight | 97 kDa |
| GenBank accession number | BC017045 |
| Gene Symbol | PYGB |
| Gene ID (NCBI) | 5834 |
| RRID | AB_3745093 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11216 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The three main subtypes of GP found in human tissues-brain-type glycogen phosphorylase (PYGB), liver-type glycogen phosphorylase (PYGL), and muscle-type glycogen phosphorylase (PYGM)-differ in function, structure, and tissue distribution. PYGB (Brain‑type glycogen phosphorylase) is a glycogen phosphorylase and is primarily localized in adult brain tissues and embryo liver tissues, whose function is to provide energy to organisms (PMID: 30106110). PYGB has been observed to be overexpressed in many types of cancer and tumor cell lines and is expected to be a novel target for the diagnosis and treatment of various cancers (PMID: 38334681).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PYGB antibody 86737-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |