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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYCRL Polyclonal antibody (16008-1-AP)

    WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PYCRL Polyclonal antibody (16008-1-AP)

    WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

PYCRL Polyclonal antibody, PBS Only

PYCRL Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16008-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16008-1-PBS targets PYCRL in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8716

Product name: Recombinant human PYCRL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-274 aa of BC007993

Sequence: MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK

相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase-like
Calculated molecular weight 274 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007993
Gene Symbol PYCRL
Gene ID (NCBI) 65263
RRIDAB_3669228
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53H96
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)

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