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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55060-1-AP

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 55060-1-AP

    WB analysis of HeLa using 55060-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 55060-1-AP

    WB analysis of L02 using 55060-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55060-1-AP

    IF Staining of HeLa using 55060-1-AP

    Immunofluorescent analysis of HeLa cells using 55060-1-AP (PYCR2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55060-1-AP targets PYCR2-specific in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight 34 kDa
Observed molecular weight 27-31 kDa, 20 kDa
GenBank accession numberNM_013328
Gene symbol PYCR2
Gene ID (NCBI) 29920
RRIDAB_10888635
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

Protocols

Product Specific Protocols
WB protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IF protocol for PYCR2-specific antibody 55060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Authors - Debanik Choudhury
View Article
humanWB

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones
View Article