Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse liver using 22163-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 22163-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22163-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22163-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 22163-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 22163-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse brain using 22163-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22163-1-AP (PXMP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22163-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

PEX2, 35 kDa peroxisomal membrane protein, EC:2.3.2.27, PAF1, PAF-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, mouse brain tissue
Positive IHC detected in	human liver tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 1 publications below

Product Information

22163-1-AP targets PXMP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17815 Product name: Recombinant human PXMP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-305 aa of BC000661 Sequence: VQKLKAKLSSWCIPLTGAPNSDNTLATSGKECALCGERPTMPHTIGCEHIFCYFCAKSSFLFDVYFTCPKCGTEVHSLQPLKSGIEMSEVNPL 相同性解析による交差性が予測される生物種
Full Name	peroxisomal membrane protein 3, 35kDa
Calculated molecular weight	305 aa, 35 kDa
Observed molecular weight	35-40 kDa
GenBank accession number	BC000661
Gene Symbol	PXMP3
Gene ID (NCBI)	5828
RRID	AB_2879014
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28328
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PXMP3 antibody 22163-1-AP	Download protocol
WB protocol for PXMP3 antibody 22163-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Rep Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses. Authors - Anu S Nath View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 22163-1-AP

WB analysis of mouse liver using 22163-1-AP

WB analysis of HeLa using 22163-1-AP

WB analysis of mouse brain using 22163-1-AP

IHC staining of human liver using 22163-1-AP