Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16315-1-AP targets PXK in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9362 Product name: Recombinant human PXK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC014479 Sequence: MAFMEKPPAGKVLLDDTVPLTAAIEASQSLQSHTEYIIRVQRGISVENSWQIVRRYSDFDLLNNSLQIAGLSLPLPPKKLIGNMDREFIAERQKGLQNYLNVITTNHILSNCELVKKFLDPNNYSANYTEIALQQVSMFFRSEPKWEVVEPLKDIGWRIRKKYFLMKIKNQPKERLVLSWADLGPDKYLSDKDFQCLIKLLPSCLHPYIYRVTFATANESSALLIRMFNEKGTLKDLIYKAKPKDPFLKKYCNPKKIQGLELQQIKTYGRQILEVLKFLHDKGFPYGHLHASNVMLDGDTCRLLDLENSLLGLPSFYRSYFSQFRKINTLESVDVHCFGHLLYEMTYGRPPD 相同性解析による交差性が予測される生物種 |
| Full Name | PX domain containing serine/threonine kinase |
| Calculated molecular weight | 450aa,52 kDa; 578aa,65 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC014479 |
| Gene Symbol | PXK |
| Gene ID (NCBI) | 54899 |
| RRID | AB_3669237 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z7A4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PXK is part of the sorting nexin (SNX) family of proteins, which are important for receptor internalization, organelle trafficking including endosomal trafficking, and other membrane-centric sorting functions. This is accomplished primarily through the PX-domain mediated binding of PI3P . PXK was first identified and cloned by two independent groups in 2005. Initial studies established that PXK is detectable in most tissues with a primarily cytoplasmic distribution. (PMID: 25620976)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PXK antibody 16315-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |