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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PWP2 Polyclonal antibody (11390-1-AP)

    WB analysis of HeLa using 11390-1-AP (same clone as 11390-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11390-1-AP (PWP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11390-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PWP2 Polyclonal antibody (11390-1-AP)

    IP experiment of HeLa using 11390-1-AP (same clone as 11390-1-PBS)

    IP result of anti-PWP2 (IP:11390-1-AP, 4ug; Detection:11390-1-AP 1:600) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 11390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PWP2 Polyclonal antibody (11390-1-AP)

    IF Staining of HepG2 using 11390-1-AP (same clone as 11390-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using PWP2 antibody 11390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11390-1-PBS in a different storage buffer formulation.

PWP2 Polyclonal antibody, PBS Only

PWP2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11390-1-PBS targets PWP2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1936

Product name: Recombinant human PWP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 520-919 aa of BC014988

Sequence: SWDKTVRLWDMFDSWRTKETLALTSDALAVTIRPDGAELAVATLNSQITFWDPENAVQTGSIEGRHDLKTGRKELDKITAKHAAKGKAFTALCYSADGHSILAGGMSKFVCIYHVREQILMKRFEISCNLSLDAMEEFLNRRKMTEFGNLALIDQDAGQEDGVAIPLPGVRKGDMSSRHFKPEIRVTSLRFSPTGRCWAATTTEGLLIYSLDTRVLFDPFELDTSVTPGRVREALRQQDFTRAILMALRLNESKLVQEALEAVPRGEIEVVTSSLPELYVEKVLEFLASSFEVSRHLEFYLLWTHKLLMLHGQKLKSRAGTLLPVIQFLQKSIQRHLDDLSKLCSWNHYNMQYALAVSKQRGTKRSLDPLGSEEEAEASEDDSLHLLGGGGRDSEEEMLA

相同性解析による交差性が予測される生物種
Full Name PWP2 periodic tryptophan protein homolog (yeast)
Calculated molecular weight 919 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC014988
Gene Symbol PWP2
Gene ID (NCBI) 5822
RRIDAB_2284709
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15269
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The function of PWP2 remains largely unknown. Catalog#11390-1-AP is a rabbit polyclonal antibody raised against the full-length of human PWP2. The MW of this protein is 102 kDa, and this antibody specially recognises the 102 kDa protein.

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