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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PVRIG Polyclonal antibody (21448-1-AP)

    WB analysis using 21448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21448-1-AP (PVRIG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PVRIG Polyclonal antibody (21448-1-AP)

    IHC staining of human tonsillitis using 21448-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PVRIG Polyclonal antibody

PVRIG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21448-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein PVRIG, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R, CD112 receptor, C7orf15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMOLT-4 cells, NK-92 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21448-1-AP targets PVRIG in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13815

Product name: Recombinant human PVRIG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 193-326 aa of BC001129

Sequence: LRRHKHRPAPRLQPSRTSPQAPRARAWAPSQASQAALHVPYATINTSCRPATLDTAHPHGGPSWWASLPTHAAHRPQGPAAWASTPIPARGSFVSVENGLYAQAGERPPHTGPGLTLFPDPRGPRAMEGPLGVR

相同性解析による交差性が予測される生物種
Full Name poliovirus receptor related immunoglobulin domain containing
Calculated molecular weight 326 aa, 34 kDa
Observed molecular weight ~34 kDa
GenBank accession numberBC001129
Gene Symbol PVRIG
Gene ID (NCBI) 79037
RRIDAB_2878859
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DKI7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer.

Protocols

Product Specific Protocols
IHC protocol for PVRIG antibody 21448-1-APDownload protocol
WB protocol for PVRIG antibody 21448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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